Return to Main Media Recipes Page Natural Seawater Black Sea Medium F/2 MN

Black Sea Medium
filtered seawater	738 ml
ddH2O	262 ml
NaNO3 (f/2 working stock)	1 ml
NaH2PO4.H2O (f/2 working stock)	1 ml
Trace Metals (f/2 working stock)	1 ml
F/2 Vitamins (f/2 working stock)	0.5 ml
NH4Cl (see below)	1 ml
Black Sea Working Stock
NH4Cl	134 mg/50 ml

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F/2
Ingredient	ml working stock/L filtered seawater
NaNO3	1
NaH2PO4.H2O	1
Trace Metals (see below)	1
Na2SiO3.9H2O	1
F/2 Vitamins (see below)	0.5

F/2 Working Stocks
NaNO3	75 g/liter dH2O
NaH2PO4.H2O	5 g/liter dH2O
Na2SiO3.9H2O	30 g/liter dH2O
F/2 Primary Trace Metal Stocks
CuSO4.5H2O	0.98 g/100ml
ZnSO4.7H2O	2.2 g/100ml
CoCl2.6H2O	1.0 g/100ml
MnCl2.4H2O	1.8 g/100ml
NaMoO4.2H2O	0.63 g/100ml
F/2 Working Trace Metal Stock
	Add to 1 liter dH2O
Na2EDTA	4.36 g
FeCl3.6H2O	3.15 g
primary trace metal stocks (5 in all):	1 ml each
F/2 Primary Vitamin Stocks
Vitamin B12	1 mg/ml
Biotin	0.1 mg/ml
F/2 Working Vitamin Stock
	Add to 1 liter dH2O
Biotin primary stock	10.0 ml
Vitamin B12 primary stock	1.0 ml
Thiamine HCl	200 mg

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MN
Ingredient	mg/L filtered seawater
Disodium EDTA	0.5
Citric acid	3
NaNO3	750
NaH2PO4.H2O	20
MgSO4.7H2O	38
CaCl2.2H2O	18
Na2CO3.(H2O)	20
SL-8 Micronutrient Solution (See Below)	1 ml
Ferric ammonium citrate	3
Rippka, R., J. Deruelles, J.B. Waterbury, M. Herdman, and R.Y. Stanier. 1979. J. Gen. Microbiol. 111, 1.

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SL-8 Micronutrient Solution
Ingredient	g/L ddH2O
Na2-EDTA	5.20
FeCL2.4H2O	1.50
ZnCl2	0.07
MnCl2.4H2O	0.10
H3BO3	0.062
CoCl2.6H2O	0.19
CuCl2.2H2O	0.017
NiCl2.6H2O	0.024
NaMoO4.2H2O	0.036
Pfenning N., and H. G. Truper. 1981. Isolation of Members of the Families Chromatiaceae and Chlorobiaceae, p. 279-289. In M. P. Starr, H. Stolp, H. G. Truper, A. Balows, and H. G. Schlegel (eds.), The Prokaryotes. , vol. 1. Springer-Verlag, Berlin, Heidelberg, New York.

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