Return to Main Media Recipes Page D & Derivatives D DTN ND NT

D
Ingredient	mg/L
Nitrilotriacetic acid (NTA)	100
NaNO3	700
KNO3	100
Na2HPO4	110
MgSO4.7H2O	100
CaSO4.2H2O	60
NaCl	8
FeCl3	0.3
D Micronutrients (See Below)	0.5 ml
Prepare this solution as a 20-fold concentrated stock and store at 4 oC. Adjust the pH to 8.2 before autoclaving.
Castenholz, R. W. 1988. Culturing methods for cyanobacteria. In L. Packer and A. N. Glazer, (eds.) Cyanobacteria Methods in Enzymology, 167: 68-100.

 
 

D Micronutrients
Ingredient	g/L
H2SO4 (conc)	0.5 ml
H3BO3	0.5
MnSO4.H2O	2.28
ZnSO4.7H2O	0.5
CuSO4.5H2O	0.025
Na2MoO4.2H2O	0.025
NiSO4(NH4)2SO4.6H2O	0.019
Na2SeO4	0.004
CoCl2.6H2O	0.045
Castenholz R. W. 1981. Isolation and Cultivation of Thermophylic Cyanobacteria, p. 237-246. In M. P. Starr, H. Stolp, H. G. Truper, A. Balows, and H. G. Schlegel (eds.), The Prokaryotes. , vol. 1. Springer-Verlag, Berlin, Heidelberg, New York.

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DTN
Ingredient
D	1 L
Tricine buffer	0.8 g
NH4Cl	0.2 g
Add ingredients to D medium and adjust the pH to 8.2 before autoclaving to make DTN medium.

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ND (D minus combined inorganic Nitrogen)
Ingredient	mg/L
Nitrilotriacetic acid (NTA)	100
Na2HPO4	70
MgSO4.7H2O	100
CaSO4.2H2O	60
NaCl	8
FeCl3	0.3
KH2PO4	36
D Micronutrients	0.5 ml
Castenholz, R. W. 1988. Culturing methods for cyanobacteria. In L. Packer and A. N. Glazer, (eds.) Cyanobacteria Methods in Enzymology, 167: 68-100.

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NT
Ingredient
ND	1 L
(NH4)2SO4	0.1 g
Na2S2O3.5H2O	0.1 g
Travertine powder	1 g
Add ingredients to 1 liter ND to make 1 liter NT. Adjust the pH to 8.2 and autoclave.

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