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BG-11 & Derivatives

BG-11

Ingredient

mg/L ddH2O

Disodium EDTA

1

Citric Acid

6

NaNO3

1500

K2HPO4. 3H2O

40

MgSO4.7H2O

75

CaCl2.2H2O

36

Na2CO3(H2O)

20

Ferric ammonium citrate

6

SL-8 Micronutrient Solution (See Below)

1 ml

DN vitamin mix (See Below)

1 ml

Prepare this solution as a 20-fold concentrated stock and store frozen. Adjust the pH to 8.2 before autoclaving.

Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazier. 1971. Purification and Properties of Unicellular Blue-Green Algae (Order Chroococcales), Bacteriological Reviews, Vol. 35, No. 2, 171-205.
 
  

SL-8 Micronutrient Solution

Ingredient

g/L ddH2O

Na2-EDTA

5.20

FeCL2.4H2O

1.50

ZnCl2

0.07

MnCl2.4H2O

0.10

H3BO3

0.062

CoCl2.6H2O

0.19

CuCl2.2H2O

0.017

NiCl2.6H2O

0.024

NaMoO4.2H2O

0.036

Pfenning N., and H. G. Truper. 1981. Isolation of Members of the Families Chromatiaceae and Chlorobiaceae, p. 279-289. In M. P. Starr, H. Stolp, H. G. Truper, A. Balows, and H. G. Schlegel (eds.), The Prokaryotes. , vol. 1. Springer-Verlag, Berlin, Heidelberg, New York.
 
  

DN Vitamin Mix

Ingredient

mg/mL-1

Nicotinic acid

0.100

PABA

0.010

Biotin

0.001

Thiamin

0.200

Cyanocobalamin

0.001

Folic acid

0.001

myo-Inositol

0.001

Calcium pantothenate

0.100

Nelson, D. C., J. B. Waterbury, and H. W. Jannasch. 1982. Arch. Microbiol. 133, 172.

 

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IOBG-11 (Instant Ocean BG-11)

Ingredient

BG-11

1 L

Instant Ocean

25 g

Add 25g Instant Ocean per liter BG11 before adjusting the pH to 8.2 and autoclaving.

 

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43.5 0/00 IOBG-11

Ingredient

IOBG-11

1 L

NaCl

19.5 g

Add 19.5 g NaCl per liter IOBG11 before adjusting the pH to 8.8 and autoclaving.

 

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70 0/00 IOBG-11

Ingredient

IOBG-11

1 L

NaCl

46 g

Add 46 g NaCl per liter IOBG11 before adjusting the pH to 8.8 and autoclaving.

 

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