Return to Main Media Recipes Page BBM & Derivatives BBM (Bold's Basal Medium) B+E Trebouxia

BBM (Bold's Basal Medium)
Ingredient	Working Stock g/L	ml working stock/ L ddH2O
NaNO3	25	10
CaCl2.2H2O	2.5	10
MgSO4.7H2O	7.5	10
K2HPO4 (Dibasic)	7.5	10
KH2PO4	17.5	10
NaCl	2.5	10
FeSO4.7H2O	4.98	1
H3BO3	11.42	1
KOH Soln.	See Below	1
Trace Metals	See Below	1
Add working stock to ddH2O to a final volume of 1 liter and autoclave.

 
 

BBM KOH Solution
Ingredient	g/100 ml
KOH	3.1
EDTA	5

 
 

BBM Trace Metals
Ingredient	g/ 100 ml
ZnSO4.7H2O	0.882
MnCl2.4H2O	0.144
MoO3	0.070
CuSO4.5H2O	0.157
Co(NO3)2.6H2O	0.049

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B+E
Ingredient
Soil water supernatant	40 ml
BBM	960 ml
Add 40 ml soil water supernatant to 960 ml BBM to make 1 liter B+E.

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Trebouxia
Ingredient
BBM	850 ml
Soil water supernatant	140 ml
Proteose peptone	10 g
Glucose	20 g

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